Plasmid_Backbone

Part:BBa_K812000:Design

Designed by: William Rostain, Tristan Cerisy   Group: iGEM12_Evry   (2012-09-18)


Biobricked pCS2+ plasmid for frogs and chicken


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4092
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 248
    Illegal NotI site found at 4098
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4092
    Illegal BamHI site found at 4077
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4092
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4092
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4107
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2611
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 3532
    Illegal BsaI.rc site found at 1634
    Illegal SapI site found at 551


Design Notes

The biobrick site has been inserted in order to obtimize the expression of the protein.


Source

This part is the biobricked version of a standard plasmid (see http://sitemaker.umich.edu/dlturner.vectors/home). We obtained this version from Nicolas Pollet lab and it has been biobricked by our team.

References